Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Abstract

Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.

Abstract of "Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor" by Park SJ, Jeong TY, Shin SK et al., https://doi.org/10.1186/s13059-021-02389-w, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.