Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains

Abstract

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.

Abstract of "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains" by Isaksson L, Mayzel M, Saline M et al., https://doi.org/10.1371/journal.pone.0062947, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.