Synthetic Biology & Genome Engineering
Synthetic genomes, genetic circuits, cell-free systems, genome writing and biomanufacturing, plus biosafety and governance considerations.
Most influential
- Synthetic biology: applications come of ageReview1,381 citationsdoi:10.1038/nrg2775
- A synchronized quorum of genetic clocksArticle995 citationsdoi:10.1038/nature08753
- Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers
Virus-like particles (VLPs) are virus-derived structures made up of one or more different molecules with the ability to self-assemble, mimicking the form and size of a virus particle but lacking the genetic material so they are not capable of infecting the host cell. Expression and self-assembly of the viral structural proteins can take place in various livi…
Abstract excerpt by Nooraei S et al., CC-BY, https://doi.org/10.1186/s12951-021-00806-7; truncated.
Review623 citationsdoi:10.1186/s12951-021-00806-7 - Protein-Sol: a web tool for predicting protein solubility from sequence
Motivation Protein solubility is an important property in industrial and therapeutic applications. Prediction is a challenge, despite a growing understanding of the relevant physicochemical properties. Results Protein-Sol is a web server for predicting protein solubility. Using available data for Escherichia coli protein solubility in a cell-free expression …
Abstract excerpt by Hebditch M et al., CC-BY, https://doi.org/10.1093/bioinformatics/btx345; truncated.
Article641 citationsdoi:10.1093/bioinformatics/btx345 - Paper-Based Synthetic Gene NetworksArticle703 citationsdoi:10.1016/j.cell.2014.10.004
- Engineering bacteria as interactive cancer therapiesReview456 citationsdoi:10.1126/science.add9667
- Genetic programs constructed from layered logic gates in single cellsArticle581 citationsdoi:10.1038/nature11516
- Synthetic biology to access and expand nature's chemical diversityReview483 citationsdoi:10.1038/nrmicro.2015.24
- A Synthetic Optogenetic Transcription Device Enhances Blood-Glucose Homeostasis in MiceArticle469 citationsdoi:10.1126/science.1203535
- Gene-Expressing Liposomes as Synthetic Cells for Molecular Communication Studies
The bottom-up branch of synthetic biology includes – among others – innovative studies that combine cell-free protein synthesis with liposome technology to generate cell-like systems of minimal complexity, often referred to as synthetic cells. The functions of this type of synthetic cell derive from gene expression, hence they can be programmed in a modular,…
Abstract excerpt by Giordano Rampioni et al., CC-BY, https://doi.org/10.3389/fbioe.2019.00001; truncated.
Review446 citationsdoi:10.3389/fbioe.2019.00001 - Synthetic analog computation in living cellsArticle456 citationsdoi:10.1038/nature12148
- Cell-free synthetic biology: Thinking outside the cellReview427 citationsdoi:10.1016/j.ymben.2011.09.002
- Materials design by synthetic biologyReview389 citationsdoi:10.1038/s41578-020-00265-w
- Guidelines for Genome-Scale Analysis of Biological Rhythms
Genome biology approaches have made enormous contributions to our understanding of biological rhythms, particularly in identifying outputs of the clock, including RNAs, proteins, and metabolites, whose abundance oscillates throughout the day. These methods hold significant promise for future discovery, particularly when combined with computational modeling. …
Abstract excerpt by Hughes ME et al., CC-BY, https://doi.org/10.1177/0748730417728663; truncated.
Article237 citationsdoi:10.1177/0748730417728663 - Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biologyArticle426 citationsdoi:10.1038/ncomms1516
Newest work
- HELICS: Biobank-scale Conditional Synthetic Genome Generation via Latent Flow MatchingPreprintarXiv:2610.36361
- Proteoliposome Preparation and Functional Reconstitution of Human Mitochondrial Transporters Via Wheat Germ Cell-Free Expression SystemArticledoi:10.1002/cpz1.70500
- Quinoa and Peanut Extracts as Alternatives to Animal-Derived Extracellular Matrix Proteins in Cultured Meat ProductionArticledoi:10.1111/1750-3841.71509
- A proof-carrying architecture for synthetic genetic logic circuits under stochastic temporal contractsPreprintarXiv:2609.38218
- Characterization of recombinase-based genetic parts and circuits using nanopore sequencingArticledoi:10.1038/s41467-026-77605-x
- Let there be multifunctionality: Uncovering the dynamical zoo of the AC-DC genetic circuitArticledoi:10.1016/j.cels.2026.101715
- From gibson assembly to cell-free expression: A guide to recombinant plasmid and toscana virus N protein productionArticledoi:10.1016/j.jviromet.2026.115455
- Radiation-Triggered Chemokine-Secreting Probiotics for Boron Neutron Capture Therapy Based RadioimmunotherapyArticledoi:10.1002/advs.77542
- Minimal organism JCVI-syn3.0, an engineered derivative of <i>Mycoplasma mycoides</i>, encodes an assortment of unrecognized membrane transporters and immunity factorsArticledoi:10.1128/msphere.00077-26
- Optimizing RNA yield using deep neural networks coupled to massively parallel screeningPreprintarXiv:2608.23722